Bcr/Abl P210-pLEF

LOCUS       Bcr/Abl P210-pLEF               15474 bp ds-DNA     circular SYN 20-MAR-2019
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    Bcr/Abl P210-pLEF
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15474)
  AUTHORS   Kweon SM, Cho YJ, Minoo P, Groffen J, Heisterkamp N
  TITLE     Activity of the Bcr GTPase-activating domain is regulated through 
            direct protein/protein interaction with the Rho guanine nucleotide 
            dissociation inhibitor.
  JOURNAL   J Biol Chem. 2008 Feb 8;283(6):3023-30. Epub 2007 Dec 10.
  PUBMED    18070886
REFERENCE   2  (bases 1 to 15474)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Mar 20, 2019 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..15474
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        1..211
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        23..219
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      70..205
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          250..822
                     /label=-beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     830..849
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     877..896
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        877..895
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             911..1564
                     /codon_start=1
                     /product="glutathione S-transferase from Schistosoma 
                     japonicum"
                     /label=GST
                     /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
                     FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
                     GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
                     YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
     primer_bind     1522..1544
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
     CDS             1571..1588
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     promoter        complement(9734..9752)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(9735..9752)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(9772..9789)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    9778..10002
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      10048..10476
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        10490..10819
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      10670..10805
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             10886..11680
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(10940..10959)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     11550..11569
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    11854..11975
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11891..11910)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11945..11964
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(12024..12040)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12024..12040)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(12037..12059)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    12048..12064
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(12072..12102)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12117..12138
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(12255..12272)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(12426..13014)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(12506..12525)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(13185..14045)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     13808..13827
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(14046..14150)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(14225..14244)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(14718..15173)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     15299..15321
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     15313..15330
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     15314..15330
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta
       61 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
      121 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag
      181 tagtgaagag gcttttttgg aggcctaggc ttttgcaaaa agctccggat cgatcctgag
      241 aacttcaggg tgagtttggg gacccttgat tgttctttct ttttcgctat tgtaaaattc
      301 atgttatatg gagggggcaa agttttcagg gtgttgttta gaatgggaag atgtcccttg
      361 tatcaccatg gaccctcatg ataattttgt ttctttcact ttctactctg ttgacaacca
      421 ttgtctcctc ttattttctt ttcattttct gtaacttttt cgttaaactt tagcttgcat
      481 ttgtaacgaa tttttaaatt cacttttgtt tatttgtcag attgtaagta ctttctctaa
      541 tcactttttt ttcaaggcaa tcagggtata ttatattgta cttcagcaca gttttagaga
      601 acaattgtta taattaaatg ataaggtaga atatttctgc atataaattc tggctggcgt
      661 ggaaatattc ttattggtag aaacaactac atcctggtca tcatcctgcc tttctcttta
      721 tggttacaat gatatacact gtttgagatg aggataaaat actctgagtc caaaccgggc
      781 ccctctgcta accatgttca tgccttcttc tttttcctac agctcctggg caacgtgctg
      841 gttattgtgc tgtctcatca ttttggcaaa gaattgtaat acgactcact atagggcgaa
      901 ttagcttacc atgtccccta tactaggtta ttggaaaatt aagggccttg tgcaacccac
      961 tcgacttctt ttggaatatc ttgaagaaaa atatgaagag catttgtatg agcgcgatga
     1021 aggtgataaa tggcgaaaca aaaagtttga attgggtttg gagtttccca atcttcctta
     1081 ttatattgat ggtgatgtta aattaacaca gtctatggcc atcatacgtt atatagctga
     1141 caagcacaac atgttgggtg gttgtccaaa agagcgtgca gagatttcaa tgcttgaagg
     1201 agcggttttg gatattagat acggtgtttc gagaattgca tatagtaaag actttgaaac
     1261 tctcaaagtt gattttctta gcaagctacc tgaaatgctg aaaatgttcg aagatcgttt
     1321 atgtcataaa acatatttaa atggtgatca tgtaacccat cctgacttca tgttgtatga
     1381 cgctcttgat gttgttttat acatggaccc aatgtgcctg gatgcgttcc caaaattagt
     1441 ttgttttaaa aaacgtattg aagctatccc acaaattgat aagtacttga aatccagcaa
     1501 gtatatagca tggcctttgc agggctggca agccacgttt ggtggtggcg accatcctcc
     1561 aaaatcggat ctggttccgc gtggatcccc cgggctgcag gaattcgatg gccgcgccat
     1621 ggtggacccg gtgggcttcg cggaggcgtg gaaggcgcag ttcccggact cagagccccc
     1681 gcgcatggag ctgcgctcag tgggcgacat cgagcaggag ctggagcgct gcaaggcctc
     1741 cattcggcgc ctggagcagg aggtgaacca ggagcgcttc cgcatgatct acctgcagac
     1801 gttgctggcc aaggaaaaga agagctatga ccggcagcga tggggcttcc ggcgcgcggc
     1861 gcaggccccc gacggcgcct ccgagccccg agcgtccgcg tcgcgcccgc agccagcgcc
     1921 cgccgacgga gccgacccgc cgcccgccga ggagcccgag gcccggcccg acggcgaggg
     1981 ttctccgggt aaggccaggc ccgggaccgc ccgcaggccc ggggcagccg cgtcggggga
     2041 acgggacgac cggggacccc ccgccagcgt ggcggcgctc aggtccaact tcgagcggat
     2101 acgcaagggc catggccagc ccggggcgga cgccgagaag cccttctacg tgaacgtcga
     2161 gtttcaccac gagcgcggcc tggtgaaggt caacgacaaa gaggtgtcgg accgcatcag
     2221 ctccctgggc agccaggcca tgcagatgga gcgcaaaaag tcccagcacg gcgcgggctc
     2281 gagcgtgggg gatgcatcca ggccccctta ccggggacgc tcctcggaga gcagctgcgg
     2341 cgtcgacggc gactacgagg acgccgagtt gaacccccgc ttcctgaagg acaacctgat
     2401 cgacgccaat ggcggtagca ggcccccttg gccgcccctg gagtaccagc cctaccagag
     2461 catctacgtc gggggcatga tggaagggga gggcaagggc ccgctcctgc gcagccagag
     2521 cacctctgag caggagaagc gccttacctg gccccgcagg tcctactccc cccggagttt
     2581 tgaggattgc ggaggcggct ataccccgga ctgcagctcc aatgagaacc tcacctccag
     2641 cgaggaggac ttctcctctg gccagtccag ccgcgtgtcc ccaagcccca ccacctaccg
     2701 catgttccgg gacaaaagcc gctctccctc gcagaactcg caacagtcct tcgacagcag
     2761 cagtcccccc acgccgcagt gccataagcg gcaccggcac tgcccggttg tcgtgtccga
     2821 ggccaccatc gtgggcgtcc gcaagaccgg gcagatctgg cccaacgatg gcgagggcgc
     2881 cttccatgga gacgcagatg gctcgttcgg aacaccacct ggatacggct gcgctgcaga
     2941 ccgggcagag gagcagcgcc ggcaccaaga tgggctgccc tacattgatg actcgccctc
     3001 ctcatcgccc cacctcagca gcaagggcag gggcagccgg gatgcgctgg tctcgggagc
     3061 cctggagtcc actaaagcga gtgagctgga cttggaaaag ggcttggaga tgagaaaatg
     3121 ggtcctgtcg ggaatcctgg ctagcgagga gacttacctg agccacctgg aggcactgct
     3181 gctgcccatg aagcctttga aagccgctgc caccacctct cagccggtgc tgacgagtca
     3241 gcagatcgag accatcttct tcaaagtgcc tgagctctac gagatccaca aggagttcta
     3301 tgatgggctc ttcccccgcg tgcagcagtg gagccaccag cagcgggtgg gcgacctctt
     3361 ccagaagctg gccagccagc tgggtgtgta ccgggccttc gtggacaact acggagttgc
     3421 catggaaatg gctgagaagt gctgtcaggc caatgctcag tttgcagaaa tctccgagaa
     3481 cctgagagcc agaagcaaca aagatgccaa ggatccaacg accaagaact ctctggaaac
     3541 tctgctctac aagcctgtgg accgtgtgac gaggagcacg ctggtcctcc atgacttgct
     3601 gaagcacact cctgccagcc accctgacca ccccttgctg caggacgccc tccgcatctc
     3661 acagaacttc ctgtccagca tcaatgagga gatcacaccc cgacggcagt ccatgacggt
     3721 gaagaaggga gagcaccggc agctgctgaa ggacagcttc atggtggagc tggtggaggg
     3781 ggcccgcaag ctgcgccacg tcttcctgtt caccgacctg cttctctgca ccaagctcaa
     3841 gaagcagagc ggaggcaaaa cgcagcagta tgactgcaaa tggtacattc cgctcacgga
     3901 tctcagcttc cagatggtgg atgaactgga ggcagtgccc aacatccccc tggtgcccga
     3961 tgaggagctg gacgctttga agatcaagat ctcccagatc aagagtgaca tccagagaga
     4021 gaagagggcg aacaagggca gcaaggctac ggagaggctg aagaagaagc tgtcggagca
     4081 ggagtcactg ctgctgctta tgtctcccag catggccttc agggtgcaca gccgcaacgg
     4141 caagagttac acgttcctga tctcctctga ctatgagcgt gcagagtgga gggagaacat
     4201 ccgggagcag cagaagaagt gtttcagaag cttctccctg acatccgtgg agctgcagat
     4261 gctgaccaac tcgtgtgtga aactccagac tgtccacagc attccgctga ccatcaataa
     4321 ggaagatgat gagtctccgg ggctctatgg gtttctgaat gtcatcgtcc actcagccac
     4381 tggatttaag cagagttcaa aagcccttca gcggccagta gcatctgact ttgagcctca
     4441 gggtctgagt gaagccgctc gttggaactc caaggaaaac cttctcgctg gacccagtga
     4501 aaatgacccc aaccttttcg ttgcactgta tgattttgtg gccagtggag ataacactct
     4561 aagcataact aaaggtgaaa agctccgggt cttaggctat aatcacaatg gggaatggtg
     4621 tgaagcccaa accaaaaatg gccaaggctg ggtcccaagc aactacatca cgccagtcaa
     4681 cagtctggag aaacactcct ggtaccatgg gcctgtgtcc cgcaatgccg ctgagtatct
     4741 gctgagcagc gggatcaatg gcagcttctt ggtgcgtgag agtgagagca gtcctggcca
     4801 gaggtccatc tcgctgagat acgaagggag ggtgtaccat tacaggatca acactgcttc
     4861 tgatggcaag ctctacgtct cctccgagag ccgcttcaac accctggccg agttggttca
     4921 tcatcattca acggtggccg acgggctcat caccacgctc cattatccag ccccaaagcg
     4981 caacaagccc actgtctatg gtgtgtcccc caactacgac aagtgggaga tggaacgcac
     5041 ggacatcacc atgaagcaca agctgggcgg gggccagtac ggggaggtgt acgagggcgt
     5101 gtggaagaaa tacagcctga cggtggccgt gaagaccttg aaggaggaca ccatggaggt
     5161 ggaagagttc ttgaaagaag ctgcagtcat gaaagagatc aaacacccta acctggtgca
     5221 gctccttggg gtctgcaccc gggagccccc gttctatatc atcactgagt tcatgaccta
     5281 cgggaacctc ctggactacc tgagggagtg caaccggcag gaggtgaacg ccgtggtgct
     5341 gctgtacatg gccactcaga tctcgtcagc catggagtac ctggagaaga aaaacttcat
     5401 ccacagagat cttgctgccc gaaactgcct ggtaggggag aaccacttgg tgaaggtagc
     5461 tgattttggc ctgagcaggt tgatgacagg ggacacctac acagcccatg ctggagccaa
     5521 gttccccatc aaatggactg cacccgagag cctggcctac aacaagttct ccatcaagtc
     5581 cgacgtctgg gcatttggag tattgctttg ggaaattgct acctatggca tgtcccctta
     5641 cccgggaatt gacctgtccc aggtgtatga gctgctagag aaggactacc gcatggagcg
     5701 cccagaaggc tgcccagaga aggtctatga actcatgcga gcatgttggc agtggaatcc
     5761 ctctgaccgg ccctcctttg ctgaaatcca ccaagccttt gaaacaatgt tccaggaatc
     5821 cagtatctca gacgaagtgg aaaaggagct ggggaaacaa ggcgtccgtg gggctgtgag
     5881 taccttgctg caggccccag agctgcccac caagacgagg acctccagga gagctgcaga
     5941 gcacagagac accactgacg tgcctgagat gcctcactcc aagggccagg gagagagcga
     6001 tcctctggac catgagcctg ccgtgtctcc attgctccct cgaaaagagc gaggtccccc
     6061 ggagggcggc ctgaatgaag atgagcgcct tctccccaaa gacaaaaaga ccaacttgtt
     6121 cagcgccttg atcaagaaga agaagaagac agccccaacc cctcccaaac gcagcagctc
     6181 cttccgggag atggacggcc agccggagcg cagaggggcc ggcgaggaag agggccgaga
     6241 catcagcaac ggggcactgg ctttcacccc cttggacaca gctgacccag ccaagtcccc
     6301 aaagcccagc aatggggctg gggtccccaa tggagccctc cgggagtctg ggggctcagg
     6361 cttccggtct ccccacctgt ggaagaagtc cagcacgctg accagcagcc gcctagccac
     6421 cggcgaggag gagggcggtg gcagctccag caagcgcttc ctgcgctctt gctccgcctc
     6481 ctgcgttccc catggggcca aggacacgga gtggaggtca gtcacgctgc ctcgggactt
     6541 gcagtccacg ggaagacagt ttgactcgtc cacatttgga gggcacaaaa gtgagaagcc
     6601 ggctctgcct cggaagaggg caggggagaa caggtctgac caggtgaccc gaggcacagt
     6661 aacgcctccc cccaggctgg tgaaaaagaa tgaggaagct gctgatgagg tcttcaaaga
     6721 catcatggag tccagcccgg gctccagccc gcccaacctg actccaaaac ccctccggcg
     6781 gcaggtcacc gtggcccctg cctcgggcct cccccacaag gaagaagctg gaaagggcag
     6841 tgccttaggg acccctgctg cagctgagcc agtgaccccc accagcaaag caggctcagg
     6901 tgcaccaggg ggcaccagca agggccccgc cgaggagtcc agagtgagga ggcacaagca
     6961 ctcctctgag tcgccaggga gggacaaggg gaaattgtcc aggctcaaac ctgccccgcc
     7021 gcccccacca gcagcctctg cagggaaggc tggaggaaag ccctcgcaga gcccgagcca
     7081 ggaggcggcc ggggaggcag tcctgggcgc aaagacaaaa gccacgagtc tggttgatgc
     7141 tgtgaacagt gacgctgcca agcccagcca gccgggagag ggcctcaaaa agcccgtgct
     7201 cccggccact ccaaagccac agtccgccaa gccgtcgggg acccccatca gcccagcccc
     7261 cgttccctcc acgttgccat cagcatcctc ggccctggca ggggaccagc cgtcttccac
     7321 cgccttcatc cctctcatat caacccgagt gtctcttcgg aaaacccgcc agcctccaga
     7381 gcggatcgcc agcggcgcca tcaccaaggg cgtggtcctg gacagcaccg aggcgctgtg
     7441 cctcgccatc tctaggaact ccgagcagat ggccagccac agcgcagtgc tggaggccgg
     7501 caaaaacctc tacacgttct gcgtgagcta tgtggattcc atccagcaaa tgaggaacaa
     7561 gtttgccttc cgagaggcca tcaacaaact ggagaataat ctccgggagc ttcagatctg
     7621 cccggcgaca gcaggcagtg gtccagcggc cactcaggac ttcagcaagc tcctcagttc
     7681 ggtgaaggaa atcagtgaca tagtgcagag gtagcagcag tcaggggtca ggtgtcaggc
     7741 ccgtcggagc tgcctgcagc acatgcgggc tcgcccatac ccgtgacagt ggctgacaag
     7801 ggactagtga gtcagcacct tggcccagga gctctgcgcc aggcagagct gagggccctg
     7861 tggagtccag ctctactacc tacgtttgca ccgcctgccc tcccgcacct tcctcctccc
     7921 cgctccgtct ctgtcctcga attttatctg tggagttcct gctccgtgga ctgcagtcgg
     7981 catgccagga cccgccagcc ccgctcccac ctagtgcccc agactgagct ctccaggcca
     8041 ggtgggaacg gctgatgtgg actgtctttt tcattttttt ctctctggag cccctcctcc
     8101 cccggctggg cctccttctt ccacttctcc aagaatggaa gcctgaactg aggccttgtg
     8161 tgtcaggccc tctgcctgca ctccctggcc ttgcccgtcg tgtgctgaag acatgtttca
     8221 agaaccgcat ttcgggaagg gcatgcacgg gcatgcacac ggctggtcac tctgccctct
     8281 gctgctgccc ggggtggggt gcactcgcca tttcctcacg tgcaggacag ctcttgattt
     8341 gggtggaaaa cagggtgcta aagccaacca gcctttgggt cctgggcagg tgggagctga
     8401 aaaggatcga ggcatggggc atgtcctttc catctgtcca catccccaga gcccagctct
     8461 tgctctcttg tgacgtgcac tgtgaatcct ggcaagaaag cttgagtctc aagggtggca
     8521 ggtcactgtc actgccgaca tccctccccc agcagaatgg aggcagggga caagggaggc
     8581 agtggctagt ggggtgaaca gctggtgcca aatagcccca gactgggccc aggcaggtct
     8641 gcaagggccc agagtgaacc gtcctttcac acatctgggt gccctgaaag ggcccttccc
     8701 ctcccccact cctctaagac aaagtagatt cttacaaggc cctttccttt ggaacaagac
     8761 agccttcact tttctgagtt cttgaagcat ttcaaagccc tgcctctgtg tagccgccct
     8821 gagagagaat agagctgcca ctgggcacct gcgcacaggt gggaggaaag ggcctggcca
     8881 gtcctggtcc tggctgcact cttgaactgg gcgaatgtct tatttaatta ccgtgagtga
     8941 catagcctca tgttctgtgg gggtcatcag ggagggttag gaaaaccaca aacggagccc
     9001 ctgaaagcct cacgtatttc acagagcacg cctgccatct tctccccgag gctgccccag
     9061 gccggagccc agatacgggg gctgtgactc tgggcaggga cccggggtct cctggacctt
     9121 gacagagcag ctaactccga gagcagtggg caggtggccg cccctgaggc ttcacgccgg
     9181 gagaagccac cttcccaccc cttcataccg cctcgtgcca gcagcctcgc acaggcccta
     9241 gctttacgct catcacctaa acttgtactt tatttttctg atagaaatgg tttcctctgg
     9301 atcgttttat gcggttctta cagcacatca cctctttgcc cccgacggct gtgacgcagc
     9361 cggagggagg cactagtcac cgacagcggc cttgaagaca gagcaaagcg cccacccagg
     9421 tcccccgact gcctgtctcc atgaggtact ggtcccttcc ttttgttaac gtgatgtgcc
     9481 actatatttt acacgtatct cttggtatgc atcttttata gacgctcttt tctaagtggc
     9541 gtgtgcatag cgtcctgccc tgccccctcg ggggcctgtg gtggctcccc ctctgcttct
     9601 cggggtccag tgcattttgt ttctgtatat gattctctgt ggtttttttt gaatccaaat
     9661 ctgtcctctg tagtattttt taaataaatc agtgtttaca ttagcggaat tccccgggtc
     9721 tagagggccc tattctatag tgtcacctaa atgctagagc tcgctgatca gcctcgactg
     9781 tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg
     9841 aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga
     9901 gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg
     9961 aagacaatag caggcatgct ggggatgcgg tgggctctat ggcttctgag gcggaaagaa
    10021 ccagctgggg ctctaggggg tatccccacg cgccctgtag cggcgcatta agcgcggcgg
    10081 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt
    10141 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
    10201 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg
    10261 attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga
    10321 cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc
    10381 ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa
    10441 aaaatgagct gatttaacaa aaatttaacg cgaattaatt ctgtggaatg tgtgtcagtt
    10501 agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
    10561 ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
    10621 catgcatctc aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct
    10681 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
    10741 agaggccgag gccgcctctg cctctgagct attccagaag tagtgaggag gcttttttgg
    10801 aggcctaggc ttttgcaaaa agctcccggg agcttgtata tccattttcg gatctgatca
    10861 agagacagga tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg caggttctcc
    10921 ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa tcggctgctc
    10981 tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg tcaagaccga
    11041 cctgtccggt gccctgaatg aactgcagga cgaggcagcg cggctatcgt ggctggccac
    11101 gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa gggactggct
    11161 gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc ctgccgagaa
    11221 agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg ctacctgccc
    11281 attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg aagccggtct
    11341 tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg aactgttcgc
    11401 caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg
    11461 cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact gtggccggct
    11521 gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg ctgaagagct
    11581 tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc ccgattcgca
    11641 gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga gcgggactct ggggttcgaa
    11701 atgaccgacc aagcgacgcc caacctgcca tcacgagatt tcgattccac cgccgccttc
    11761 tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc
    11821 ggggatctca tgctggagtt cttcgcccac cccaacttgt ttattgcagc ttataatggt
    11881 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
    11941 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctgtatacc gtcgacctct
    12001 agctagagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc
    12061 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga
    12121 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
    12181 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
    12241 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
    12301 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
    12361 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
    12421 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
    12481 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
    12541 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
    12601 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
    12661 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
    12721 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
    12781 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
    12841 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
    12901 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
    12961 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
    13021 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
    13081 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
    13141 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
    13201 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
    13261 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
    13321 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
    13381 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
    13441 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
    13501 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
    13561 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
    13621 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
    13681 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
    13741 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
    13801 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
    13861 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
    13921 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
    13981 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
    14041 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
    14101 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
    14161 cgaaaagtgc cacctgacgt cgacggatcg ggagatctcc cgatccccta tggtgcactc
    14221 tcagtacaat ctgctctgat gccgcatagt taagccagta tctgctccct gcttgtgtgt
    14281 tggaggtcgc tgagtagtgc gcgagcaaaa tttaagctac aacaaggcaa ggcttgaccg
    14341 acaattgcat gaagaatctg cttagggtta ggcgttttgc gctgcttcgc gatgtacggg
    14401 ccagatatac gcgttgacat tgattattga ctagttatta atagtaatca attacggggt
    14461 cattagttca tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc
    14521 ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag
    14581 taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc
    14641 acttggcagt acatcaagtg tatcatatgc ggtgtgaaat accgcacaga tgcgtaagga
    14701 gaaaataccg catcaggaaa ttgtaagcgt taatattttg ttaaaattcg cgttaaattt
    14761 ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc
    14821 aaaagaatag accgagatag ggttgagtgt tgttccagtt tggaacaaga gtccactatt
    14881 aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg atggcccact
    14941 acgtgaacca tcaccctaat caagtttttt ggggtcgagg tgccgtaaag cactaaatcg
    15001 gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga acgtggcgag
    15061 aaaggaaggg aagaaagcga aaggagcggg cgctagggcg ctggcaagtg tagcggtcac
    15121 gctgcgcgta accaccacac ccgccgcgct taatgcgccg ctacagggcg cgtccattcg
    15181 ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc
    15241 cagctggcga aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc
    15301 cagtcacgac gttgtaaaac gacggccagt gaattgtcga cttctgaggc ggaaagaacc
    15361 agctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa
    15421 gtatgcaaag catgcatctc aattagtcag caaggaaagt ccccaggctc ccca
//